Integrin avβ6 Effector Reporter Cell
CBP74319
| I. Background | |
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整合素αvβ6 是由αv 和β6 亞基組成的異二聚體跨膜受體，屬于整合素家族成員，其胞 外域通過識(shí)別含 RGD（精氨酸-甘氨酸-天冬氨酸）序列的配體介導(dǎo)細(xì)胞與細(xì)胞外基質(zhì)的相 互作用，主要在上皮細(xì)胞中低表達(dá)，但在組織損傷、纖維化、炎癥及惡性腫瘤中顯著上調(diào)， 成為疾病治療的潛在靶點(diǎn)。該整合素的主要配體包括細(xì)胞外基質(zhì)蛋白如纖維連接蛋白 （Fibronectin）、層粘連蛋白（Laminin）以及潛活形式的 TGF-β，后者通過與αvβ6 結(jié)合被 激活為活性形式，驅(qū)動(dòng)纖維化和免疫抑制微環(huán)境形成；此外，αvβ6 還可作為口蹄疫病毒 （FMDV）等病原體的入侵受體。 |
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| II. Description | |
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Integrin αvβ6 Effector Reporter Cell 報(bào)告基因藥靶模型很好的模擬了體內(nèi)αvβ6 的信號(hào)轉(zhuǎn) 導(dǎo)過程，原理見下圖所示。
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| III. Introduction | |
| Expressed gene: | Integrin avβ6 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin+200 μg/ml Hygromycin B+5 μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
IV. Representative Data Figure 2. Recombinant Integrin avβ6 Effector Reporter Cell stably expressing ITGB6&ITGAV.  Figure 3. Dose Response of Recombinant Human Latent TGFβ1 in Integrin avβ6 Effector Reporter Cell (C17).  Figure 4. Inhibition of Human Latent TGFβ1-induced Reporter Activity by Integrin avβ6 Blocking Antibody in Integrin avβ6 Effector Reporter Cell(C17).  |
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Figure 5. Blocking of Human Latent TGF-β1 induced Integrin avβ6 Effector Reporter Cell(C17) Activity by Integrin avB6 Blocking Ab with GARP/Latent TGF-β1 Target Cell(C5).
Figure 6.Blocking of Human Latent TGF-β1 induced Integrin avβ6 Effector Reporter Cell(C17) Activity by GARP/TGF-β1 Blocking Ab with GARP/Latent TGF-β1 Target Cell(C5).
