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首頁 /藥靶模型 /GPCR靶點細胞 /Class A(Rhodopsin) /Human ADORA1/CHO

Human ADORA1/CHO

CBP71347

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I. Background

ADORA1 is a receptor for adenosine. It is demonstrated that the ADORA1 receptor is ubiquitous throughout the entire body. Activation of ADORA1 elicits an inhibition of adenylate cyclase and therefore a decrease in the cAMP concentration. ADORA1 receptors are implicated in sleep promotion by inhibiting wake promoting cholinergic neurons in the basal forebrain. Adenosine antagonists are widely used in neonatal medicine.

 
II. Introduction
Host Cell:

CHO

Stability: 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: F12k+10%FBS+5ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen immediately upon delivery
Application(s):

Functional assay for A1 receptor

Transducer:  Gi
 
Ⅲ. Description of Host Cell Line
Organism: Hamster
Tissue: Ovary
Morphology: Epithelial
Growth Properties: Adherent
 
Ⅳ. Representative Data

Figure 1. NECA efffect on CHO-K1-ADORA1 clone 5.


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