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首頁 /科研細胞 /人源細胞系 /胰腺 /HPAC(人胰腺癌細胞)

HPAC(人胰腺癌細胞)

CBP60539

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I. General information 
Synonyms: HPAC
Background: HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin.
Species: Homo sapiens, human
Tissue: pancreas
Disease: adenocarcinoma
Gender: Caucasian, female, 64 years
Morphology: epithelial
Growth Mode: adherent
Doubling Time: 41 hrs
DNA Profile: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,10
D5S818: 12
D7S820: 10,12
THO1: 9.3
TPOX: 10,11
vWA: 15,17
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Culture Medium:

DMEM/F12(1:1)+0.002 mg/ml insulin+0.005 mg/ml transferrin+ 40 ng/ml hydrocortisone+ 10 ng/ml epidermal growth factor +10%FBS

HPAC完全培養(yǎng)基,#CBP60539M
We strongly suggest to purchase the complete medium from us.

Cryopreservation medium: 90%FBS+10%DMSO
Receptor Expression: epidermal growth factor (EGF), expressed,glucocorticoid, expressed,epidermal growth factor (EGF); glucocorticoid
Genes Expressed:  HPAC cells are positive for keratin and negative for vimentin and chromogranin A.
Tumor Formation: Yes, the cells form tumors in athymic nude mice at the site of inoculation which are histologically similar to the tumor of origin,Yes, the cells have a colony forming efficiency of 64% on plastic and 3.2% in agarose.
Comments: HPAC proliferation is stimulated by insulin, insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), and transforming growth factor alpha (TGF alpha). Cell growth is suppressed by dexamethasone and other glucocorticoids. Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9.
In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells.
HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor. Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1.
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