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MDBK(牛腎細(xì)胞)

CBP61030

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I. General information 
Synonyms: MDBK
Background: The MDBK cell line was derived from a kidney of an apparently normal adult steer, February 18, 1957, by S.H. Madin and N.B. Darby. The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145).­
Species: Bos taurus, cow
Tissue: kidney
Gender: male,  adult
Morphology: epithelial
Growth Mode: adherent
Culture Medium:

MEM+10%HS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)

MDBK完全培養(yǎng)基,# CBP61030M
We strongly suggest to purchase the complete medium from us.

Cryopreservation medium: 90%FBS+10%DMSO
Genes Expressed: keratin
Comments: Vesicular stomatitis, Orsay (Indiana)
Infectious bovine rhinotracheitis
Bovine parvovirus
Bovine adenovirus 2
Bovine adenovirus 3
Bovine viral diarrhea virus 1 , Bovine viral diarrhea virus 1
Parainfluenza 3                                                                                                          
poliovirus 2
A specific lot of CCL-22, MDBK (NBL-1) bovine kidney cells was found to be positive for bovine viral diarrhea virus (BVDV), following an investigation by ATCC (Dec. 2015). Testing for BVDV was performed in compliance with the Code of Federal Regulations, Title 9, Section 113.52 (E &F) using virus-specific fluorescent antibody (FA) technique as well as non-specific tests for hemadsorption and cytopathic effects (CPE). Samples were found to be positive for BVDV by FA. Hemadsorption and CPE were not observed in the sample inoculated cultures.  As a result,  the BSL status for CCL-22 was changed to “2”. 
Subsequent lots of CCL-22, MDBK (NBL-1) bovine kidney cells are tested for BVDV and the results will be indicated on the Certificate of Analysis for the lot.
The cells are positive for keratin by immunofluorescence
For more information, please contact us (4008-750-250).

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藥靶模型聯(lián)系方式: 華東銷售經(jīng)理(上海):18240630236 華東銷售經(jīng)理(上海、江蘇、安徽):15715191010 華中&華西銷售經(jīng)理:18071545918 華中&西南銷售經(jīng)理:13871580511 華北銷售經(jīng)理:18628311252 全國銷售經(jīng)理:13816461235
診斷標(biāo)準(zhǔn)品聯(lián)系方式: 華東銷售經(jīng)理:15000320447 華北銷售經(jīng)理:18628311252 華中&華西銷售經(jīng)理:18071545918 華中&西南銷售經(jīng)理:13871580511 全國銷售經(jīng)理:13816461235

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